Chaning waste into an energy source

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There is 2-possible ways of inserting a gene into E.Coli that functions in changing a waste into an energy source.
5` to 3` direction and 3` to 5` direction. Which of these two would allow the E. Coli to carry out the function of changning waste products to energy source. Chaning waste into an energy source is just a hypothetical function.

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Chaning waste into an energy source : There is 2-possible ways of inserting a gene into E.Coli that functions in converting a waste into an energy source.

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