Cellulase is the enzyme

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Cellulase (EC 3.2.1.4) is the enzyme.

1) What is the source of this enzyme (organism).

Chose a sequence of your amino acid and provide information about the sequence you have chosen. You must explain the code (EC number) of your enzyme.

2) Explain the reaction that the enzyme catalyzes with the help of a reaction diagram.

3) Calculate the charge of the protein at pH 1, pH 7 and pH 14.

4) The isoelectric pH (pI) is the pH where the molecule has no charge (neutral).

a) Explain how to estimate the pI of this protein.

b) Estimate the pI of this protein (show your calculations)

c) Explain a way to experimentally obtain the exact pI of a protein.

Reference no: EM133261173

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