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Cell concentration in the outlet stream

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Reference no: EM132052233

A CHO mammalian cell culture is set up by a chemostat stirred tank bioreactor to produce HBsAg. Glucose is the growth rate limited substrate and cell growth follows the Monod Model.

The feeding flow rate is 20L/hr with the glucose concentration 0.25M, and the glucose concentration in the outlet is 5g/L. It has been found out the maximal cell growth rate is 0.2 hr-1 and the saturation constant, or half-velocity constant of substrate is 2.0 g/L. The cell yield coefficient is Y X/S = 0.30g/g. Find out:

  1. CHO cell concentration in the outlet stream.
  2. Culture medium volume in the bioreactor at flow rate of 20L/hr.
  3. Keep the culture medium volume from b) constant, what is maximal flow rate to avoid the cells washed out from this bioreactor?

Reference no: EM132052233

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