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You want to fuse a cDNA sequence to the end of two tandem"HA" antibody epitope tags within the MCS-HAHA (multiple cloning site-HAHA) region of the vector shown below. When transformed into yeast this plasmid will express the fusion protein under the transcriptional control of the pGAL1 promoter as shown. The corresponding sequence of the MCS-HAHA region is shown from the EcoRI site to the Sal1 site, and the reading frame of the two HA tags is indicated (each HA epitope is designated by the underlined amino acid sequence). Design primers with XhoI and SalI sites for PCR amplification of the entire cDNA (sequence at the bottom) to clone it together, in frame, with the HAHA epitope in the vector. Calculate for both primers their respective Tm and report the sequence of temperatures and times required for one cycle of the PCR amplification.
plan the structure of butanol and show water molecules in hydrogen bonds to the butanol. How many should there be? remember the H bonding rules.
Point out similarities and differences in their scientific foundation. What is the mainly abundant cell type? Which is the slightest prevalent.
Based on the general permeability equation, net flux of solute across a cell membrane, Jnet (gm/sec), is proportional to the permeability coefficient, P,
If there were 200 F2 offspring approximately how many would you expect to be both red eyed and shy. If an F1 Nessie were to be mated to a blue eyed and outgoing Nester what would be expected phenotypic ratio of the off spring use a punnett square.
What is the estimated efficiency of cellular respiration for a mutant organism that produces only 29 mole of ATP for every mole of glucose oxidized, sooner than the usual 36-38 moles of ATP.
Monarch butterfly populations are found east and west of Rocky Mountains. How can DNA fingerprinting technology be used to establish that they constitute one breeding population or two separate breeding populations.
find which of the following describes the type of the reaction(s) catalyzed: condensation (carbon-carbon bond formation); dehydration (loss of water); hydration (addition of water); decarboxylation (loss of CO2); oxidation-reduction; sutrate-level..
Explain the events that occur when epinephrine binds to a G-protein coupled receptor in the membrane of a cell. Begin with the binding of the epinephrine and end with the activation of adenylyl cyclase.
The research team used the setup to study the incorporation of labelled nucleotides into culture lymphocytes and found that the lymphocytes incorporated the labelled nucleotide at significantly higher level after a pathogen was introduced into the cu..
Approximately what percentage of the energy of glucose is transferred to storage in ATP as the result of the complete oxidation of glucose to CO and water in cellular respiration.
During gym class Sally noticed that her friend Melissa always ran faster than her. Sally knew that they exercised equally, so she wondered what could cause Melissa to run so fast.
Both endocrine and nervous system are major regulating systems of the body; however, the nervous system has been compared to any post mail delivery system and the endocrine system to the pony express. In brief explain this comparison.
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