Calculate the true vmax and km

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Reference no: EM132296669

2309_figure.jpg

Using the above graph and the following values, solve the following questions without inhibitor, y-intercept = 2, x-intercept = -10

with 1 mM inhibitor, y-intercept = 3, x-intercept = -7.5

From these values, you should be able to calculate the "true" Vmax and Km, the values of alpha and alpha', and the values of Ki and Ki'. Based upon the comparison of Ki and Ki', you ought to be able to answer the question of whether the inhibitor binds more tightly to free enzyme or the enzyme-substrate complex.

You may assume Vmax has units of mM/sec and Km has units of mM

Reference no: EM132296669

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