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E. coli were transformed with 50 ng of plasmid DNA is a total volume of 500 µl, and 100 µl of the transformation reaction was plated on a LB amp agar plate. After overnight incubation at 37C.
Count the colonies and calculate the transformation efficiency for this experiment.
A volume of aqueous hydrochloric acid containing 0.500 mol of HCl and 0.100 mol of magnesium metal were combined and reacted to completion. How many liters of hydrogen gas were produced under STP conditions?
If an enzyme with no inhibitor has a Vmax of .091 abs/min and a Km of .31 mM. An inhibitor is added (1 uM) which changes the Vmax to .036 abs/min and a Km of .13 mM, so it has a Ki of -1.72 and a Ki' of .65 uM.
A student argued that this is too time-consuming as the complete amino acid sequence should be determined in order to create the probe. Does the student have a valid argument? Why or why not with reason.
Determine the probability that two grandchildren of the woman who are first cousins to one another will each inherit the dominant allele?
Explain in molecular detail how scientists could develop antibiotics that contact multiple sites on rRNAs and /or r-proteins within the bacterial ribosome.
Explain how incomplete penetrance, variable expressivity, pleiotropy, and genetic heterogeneity can affect the severity of a disease in a family.
Find out the genotypes of the two black females and the brown male using allele symbols B and b. One shows linear carbon chains, another has one -CH3 groups present on the third carbon atom, and third has two -CH3 groups present on the second carbon ..
Determine the suitable measure of the association between alcohol use and CHD in both the smokers and the non-smokers.
What Are the Types and Functions of Blood Vessels.
In corn three dominant genes are necessary for aleurone color. The genotype B__D__R is colored. Any homozygous recessive for one gene is colorless. Predict the genotypes and phenotypes of the offspring of the cross BbDdRR x BbDdRR.
Determine what sequence of nucleotide pairs in a Drosophila gene will encode the amino acid sequence met-trp-phe-trp-met.
If you were to create a mutant lysosomal protein in which a glutamine replaced an asparagine contained in the wild type protein the mutant has a Gln where the normal protein has an Asn.
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