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The reduced form of nicotinamide adenine dinucleotide (NADH) is an important and highly fluorescent coenzyme. It has an absorption maximum of 340 nm and an emission maximum at 465 nm. Standard solutions of NADH gave the following fluorescence intensities:
(a) Construct a spread sheet and use it to draw a calibration curve for NADH.
(b) Find the least-squares slop and intercept for the plot in (a).
(c) Calculate the standard deviation of the slope and the standard deviation about regression for the curve.
(d) An unknown exhibits a relative fluorescence intensity of 12.16. Use the spread sheet to calculate the concentration of NADH.
(e) Calculate the relative standard deviation for the result in part (d).
(f) Calculate the relative standard deviation for the result in part (d) if a result of 7.95 was the mean of three measurements
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How much heat energy is required to raise the temperature of 0.365kg of copper from 23.0C to 60.0C. The specific heat of copper is0.0920 cal/(gC).
We place the 2.00M HCl into the buret tube. Next we place 30.0 mL of the KOH unknown Molarity solution into a small flask and add some phenolphthalein indicator turning the solution an intense pink color. When we titrate exactly 32.0 mL of the 2.0..
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Can anyone explain the intermediate compound formation theory in detail
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You are titrating an unknown acid with a 0.228M NaOH standard solution. It requires 19.1 mL of the NaOH solution to reach the endpoint of the titration.? How many moles of H+ reacted with the OH- from the NaOH in the titration
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When combining with nometallic atoms, metallic atoms generally will... a) lose electrons and form negative ions b)lose electrons and form positive ions c) gain electrons and form negative ions d) gain electrons and form positive ions
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