Calculate the sedimentation coefficient

Reference no: EM13498886

Exercise 1

The sedimentation of bovine serum albumin (dBsA = 1.106 g/cm3) was measured in water at 298 K at a spinning speed of 56 850 rpm.

872_Calculate the sedimentation coefficient.png

Calculate the sedimentation coefficient, the molar mass and the frictional coefficient of the BSA given a diffusion coefficient of 6.9 x 10^-121U2/S. State any assumptions.

Exercise 2

The sedimentation and diffusion coefficients for myoglobin in dilute aqueous solution at 20 °C are 2.04 x 10^-13s and 1.13 x 10-1° m2 sel,
respectively. The partial specific volume of the protein is 0.741 cm3 g-1, the density of the solution is 1.00 g cm-3 and the coefficient of viscosity of the solution is 1.00 x 10-3 kg m^-1.s^-1. Calculate (a) the relative molecular mass and (b) the frictional ratio of this protein.

Exercise 3:

At 20.0 0C, a polymer (M = 170. kg/mol) having a specific volume of 0.773 cm3/g is centrifuged in water (density of water = 0.998 g/cm3).
Calculate the relative concentration of the polymer at r - 7.0 cm vs. r - 6.0 cm if the centrifuge is turning at 45000 rpm.

Exercise 4:

In an electrophoresis experiment, spherical colloidal particles of diameter 0.5 1-1.M are dispersed in a solution of 0.10 mon NaCl. If the particles are observed to cover a distance of 120 1.1M in 5.0 s, calculate an estimate of the error in the electrophoresis measurement arising from the Brownian displacement of the particle that occurs during the experiment.

Reference no: EM13498886

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