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Exercise 1
The sedimentation of bovine serum albumin (dBsA = 1.106 g/cm3) was measured in water at 298 K at a spinning speed of 56 850 rpm.
Calculate the sedimentation coefficient, the molar mass and the frictional coefficient of the BSA given a diffusion coefficient of 6.9 x 10-12 1U2/S. State any assumptions. Exercise 2
The sedimentation and diffusion coefficients for myoglobin in dilute aqueous solution at 20 °C are 2.04 x 10-13 s and 1.13 x 10-1° m2 sel,respectively. The partial specific volume of the protein is 0.741 cm3 g-1, the density of the solution is 1.00 g cm-3 and the coefficient of viscosity of the solution is 1.00 x 10-3 kg m-1.s-1. Calculate (a) the relative molecular mass and (b) the frictional ratio of this protein.
Exercise 3:
At 20.0 0C, a polymer (M = 170. kg/mol) having a specific volume of 0.773 cm3/g is centrifuged in water (density of water = 0.998 g/cm3).Calculate the relative concentration of the polymer at r - 7.0 cm vs. r - 6.0 cm if the centrifuge is turning at 45000 rpm.
Exercise 4: In an electrophoresis experiment, spherical colloidal particles of diameter 0.5 1-1.M are dispersed in a solution of 0.10 mon NaCl. If the particles are observed to cover a distance of 120 1.1M in 5.0 s, calculate an estimate of the error in the electrophoresis measurement arising from the Brownian displacement of the particle that occurs during the experiment.
Show all the steps in the mechanism for the following reaction, When benzene is mixed with deuterated sulfuric acid, deuterium is slowly incorporated onto the ring. Show the mechanism for this reaction and explain how this relates the sulfonation of ..
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