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Calculate the kcat

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Reference no: EM131126253

From a kinetics experiment, the Vmax was determined to be 3200111\4.min-1. For the kinetic assay, 0.2mL, of a 0.05mg/mL solution of enzyme was used, and the enzyme has a molecular weight of 95,000 g/mole.

Assume a reaction volume of 3mL.

Calculate the kcat (in • sec-1) and catalytic efficiency ( in M-1sec-1) for the enzyme.

The Km for the enzyme was determined to be 2.5 x 10-2M.

Reference no: EM131126253

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