Calculate the fraction of the total enzyme active sites

Reference no: EM13652303

Suppose that an enzyme uses a catalytic mechanism in which a functional group on the enzyme has to accept a proton from the intermediate formed in the first chemical step. The pKa of that basic functional group on the enzyme is 8.0, and is the only pKa apparent on a plot of kcat vs. pH for the activity of this enzyme. The kcat of the fully active form of the enzyme is 1 x 10^5 sec-1 .

1. Calculate the fraction of the total enzyme active sites in solution that would be in the active form at pH 7.2.

Reference no: EM13652303

Questions Cloud

Domain model class diagram : Data Flow Diagram - Context Diagram and Level and Use Case Diagram and Domain Model Class Diagram

Completely around the loop on the track : What is the height that the ball needs to be dropped from to make it completely around the loop on the track? (neglect air resistance and friction)

Determine the anchoring forces rx and ry required : Determine the anchoring forces Rx and Ry required to hold in place a conical nozzle at the end of hose when the water flowrate is 50 L/min. The nozzle is oriented at a 45 degree angle as shown below. Assume that the water has a density of 1000 kg/m3.

The force that the wall exerts on the left end of the beam : A 1350 N uniform beam is attached to a vertical wall at one end and is supported by a cable at the other end. A W = 1860 N crate hangs from the far end of the beam. Calculate the magnitude of the horizontal component of the force that the wall exe..

Calculate the fraction of the total enzyme active sites : Calculate the fraction of the total enzyme active sites in solution that would be in the active form at pH 7.2.

Find the childs speed at the lowest point : A 35.0-kg child swings in a swing supported by two chains, each 2.92 m long. The tension in each chain at the lowest point is 430 N. Find the child's speed at the lowest point. Find the force exerted by the seat on the child at the lowest point.

What is the change in momentum of basebball : A 0.04-kg baseball traveling in a horizontal direction with a speed of 16 m/s hits a bat and is popped straight up with a speed of 15 m/s. What is the change in momentum (magnitude and direction) of the baseball?

What is the smallest thickness of a soap film : What is the smallest thickness of a soap film that would appear black if illuminated with 470 nm light? Assume there is air on both sides of the soap film.

What is the spring constant problem : One end of a spring is attached to a wall. A 26 {\rm N} pull on the other end causes the spring to stretch by 3.4 {\rm cm}. What is the spring constant?

User Account

All Pages