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A crude cell-free extract of a skeletal muscle contained 32 mg protein/ml. Ten ul (microlitres) of the extract catalyzed a reaction at rate of 0.14 umole/min under standard optimum assay conditions. Fifty ml of the extract were fractionated by ammonium sulphate precipitation. The supernatant obtained after centrifugation was redissolved in 10ml. This solution was found to contain 50 mg protein/ml. Ten ul (microlitres) of this purified fraction catalyzed the reaction at a rate of 0.65 umole/min.
(a) Calculate the percent recovery of the enzyme in the purified fraction. (Yield = units of enzyme in fraction / units of enzyme in original fraction).
(b) Calculate the degree of purification obtained by the ammonium sulphate fractionation. (Specific activity = total units of enzyme in fraction / total amount of protein in a fraction)(Fold Purification = specific activity of a fraction / specific activity of original fraction).
What technique below is often used to identify transmembrane segments of integral proteins? What kind of enzyme removes a phosphate groups from a substrate for the purpose of activating or deactivating them?
How might the initial reaction occur in this pathway. as lactate is a "dead end" product of metabolism in the sense that its sole fate is to be converted into pyruvate, what is the purpose of its formation.
how to Analysis of base composition from DNA. Using this data predict the % of adenine. If the same were detected in Tobacco Mosaic Virus [TMV], will it be possible to predict the % of adenine. This job gives reasons.
Metallic magnesium has a hexagonal close-packed structure and a density of 1.74 g/cm 3 . Suppose magnesium atoms to be spheres of radius, r.
In August 2001, a man walking his dog in Lindley Woods, near Otley, in West Yorkshire, discovered the body of 16-year old Leanne Tiernan, buried in a shallow grave.
Diversity in tropical regions is generally greater than at high latitudes. Explain two different hypotheses that propose the mechanism for this pattern that remains a biological mystery
The graph demonstrate the number of accurate copies of DNA produced by polymerase chain reaction. What can you conclude about cycles 18–26?
Discuss similarities and differences of homeostatic mechanisms for thermoregulation in ectothermic and endothermic animals.
Recently, use of cleaner fuels has greatly reduced soot in the landscape, and dark-coloured moths have been disappearing. Should the two forms of moths be considered separate species.
suppose a single gene controls soybean seed color and that you already know the nucleotide sequence of the wild type allele.
Avery, McLeod and McCarthy set out to uncover Griffith's transforming principle: in other words what is it about heat killed smooth, pathogenic Streptococcus pneumoniae that makes rough, avirulent S
Estimate the most likely type of change that occurred in the DNA (single-base substitution, insertion, deletion), the phenotypic effect of the mutation
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