Calculate the concentration of apotransferrin

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Problem- The protein apotransferrin binds two Fe(III) ions for cell transport. Consider when a 3.50 mL solution of apotransferrin is tirated with 130 microliters of a 1.30M Fe(III) solution. The initially colorless solution turns red, and the color change can be measured with a spectrophotometer. Calculate the concentration of apotransferrin present in the original 3.50 mL solution

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