Calculate the bit score to two decimal places

Reference no: EM133255948

Goal: Calculate the bit score (S') to two decimal places based on the raw score S.

Given: The BLASTP query sequence AAA52280.1.

Given: The equation S' = (λS - lnK)/ln2

Search #1: Use all defaults but limit to reference proteins (refseq_proteins) and limit the organism to archaea (taxid:2157).

Search #2: Same as search #1 except flip the matrix to PAM 70 and change the Gap Costs to 7/2.

Target: Examine the best match in each search.

Hint #1: Look at the alignment for the best match. Next to the bit score (S') is the raw score in parentheses. That is S.

Hint #2: For each search, click search summary to get to obtain λ and K (use the right column, not the left).
(0.5) Enter the data below. The last column should contain the S' value you calculated using the equation.

Reference no: EM133255948

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