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The transformation efficiency is defined as the quotient of the nubmer of cells transformed and the amount of input DNA in μg. In a transformation experiment, 50μl of competent cells were mixed with a 100ng og plasmid DNA. After heat-shocking in the cell, you add 450μl of media, incubate for 1 hour and spread 70 μl of the transformation reaction on to several selective plates. the following day, you examine the plates and count an average of 200 colonies. Calculate the average transformation efficiency of this.
What is the selective advantage of producing thousands of vessels and tracheids instead of one giant conduit for water transport?
A karyotype reveals that an individual is XYY
Name four examples of compounds which are classified as carbohydrates.
Think about a man who has been exercising. His arterial receptors detect a rise in his blood CO2 and send this information to the brain. He requires to increase both the rate and depth of his breathing.
If you cross a true-breeding dominant plant with a true-breeding recessive plant, what will be the phenotype of the resulting progeny?
Suggest two methods for positively identifying carbon dioxide released in anaerobic respiration?
Describe in detail the three phases of hemostasis. How could a lack of a vitamin K injection at birth lead to increased bruising and hemorrhage?
Explain the process of the Meiosis and the Sexual Life Cycles. Eukaryotic cells may divide through the mitosis or meiosis. In humans, mitosis generates new cells for the growth and repair.
You were given a pea plant with long stems and axial flowers and are asked to determins its genotype. You know that the allele for long stem is dominant over short and the allele for axial flowers is dominant over terminal flowers. Describe the on..
In rats, a black-tailed mutant was discovered. When it was crossed to a pink long-tailed rat, 4 offspring were black-tailed and 3 were pink tailed. Two of these black-tailed rats were crossed and they produce 6 black tailed and 3 pink-tailed rats.
The new form of life is discovered. It has a genetic code much like that of organisms on Earth except that there are five different DNA bases instead of four and the base sequences is translated as doublets instead of triplets. How various different ..
suppose a bird that eats grasshoppers moves into the prairie. How will this affect natural selection of the grasshoppers? How might this change in a drought year.
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