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Calculate the amount of stock reagent(s) and water needed to make the following solutions:
2.0L of 5.0mM NaCl (from 0.5M stock), 25mM EDTA (from 1.0M stock):
500.0mL of 3.0mM sodium citrate (from dry powder), 15mM SDS (from 50.0mM stock), 0.5% glycerol.
250mL of 0.50X TBE (from 10X stock)
Explain the role of the citric acid cycle as a central metabolic mechanism. Explain what happens to the cells' abilities to oxidize acetyl CoA when intermediates of the cycle are drained off for amino acid biosynthesis.
An individual has been bought to you with the symptoms of Klinefelter syndrome, although a karyotype analysis indicates they have forty-six chromosomes.
A new Nurse intern has been working non-stop for 15 hrs in the ER. A patient was showing serious signs of dehydration. The Nurse took action without checking with the attending physician and administered 1 liter of sterile distilled water intravenous..
G-protein-linked receptors activate G proteins by reducing the strength of GDP binding. This results in rapid dissociation of abound GDP, which is then replaced by GTP, which is present in the cytosol in much higher concentrations than GDP.
If you were to remove the ER retrieval signal (KDEL) from protein disulfide isomerase (PDI), which is normally a soluble resident of the ER lumen, where would you expect the modified PDI to be located? Why?
What happens during gastrulation? A) The three embryonic tissue germ layers form. B) The blastula forms. C) The neural tube forms. D) The somites form.
What theories were synthesized to generate the modern theory of evolution? In what ways did that change Darwin's original framework?
What is the correct order of the HIV lifecycle?
Explain how temperature affects the hemoglobin saturation curve. Discuss how does temperature affect the delivery of oxygen to the tissues?
You have recovered a strain of E. coli with a null mutation of the gene for catabolite activator protein (CAP). Which of the following do you expect to be true regarding the expression of a completely wild-type operon?
Rabbits heterozygous for Pelger trait and exhibiting that phenotype are crossed. The offspring appear in a 2:1 ratio (Pelger : normal). Explain this strange ratio.
One in 1700 Caucasian new borns have cystic fibrosis. What is the allele frequency for the normal (C) and disease (c) alleles? What are the genotype frequencies?
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