Reference no: EM132798637

1. Which of the following is organelles is matched correctly with their respective marker protein?

a. Peroxisomes and Collagenase

b. Mitochondria and Cytochrome A

c. Lysosome and Acid Phosphatase

d. None of the above

2. When do we want to use Cryo-EM?

a. To observe the sample in its native, dehydrated state

b. To observe the sample 0.005 nm resolution

c. To reveal 2D sectional images

d. To observe the sample in its hydrated state

3. Which of the following methods are used to break open plasma membranes in cells?

a. Repeated freeze-thaw

b. Placing cells in hypertonic solution

c. Sonication

d. A and C

e. All of the above

4. The image from which microscopy technique is processed by a computer algorithm to produce a clearer image in deconvolution microscopy?

a. Conventional microscopy

b. Confocal microscopy

c. two-photon excitation microscopy

d. Total internal reflection microscopy

5. A researcher wants to examine the relationship between two proteins X and Y. In order to measure the distance between X and Y he uses Fluorescence Resonance Energy Transfer (FRET) and conjugates the proteins X and Y with CFP and YFP, respectively. For this technique to work which of the following should be true of CFP and YFP?

a. The emission wavelength of CFP should be shorter than the excitation wavelength of YFP.

b. The emission wavelength of CFP should be longer than the excitation wavelength of YFP.

c. The emission wavelength of CFP should be equal to the excitation wavelength of YFP.

d. The emission wavelength of CFP should be equal to the emission wavelength of YFP.

6. Light-sheet microscopy images what type of cells?

a. Fixed and stained tissue

b. Frozen samples

c. Living tissue

d. Metal shadowed sample

7. What can be used to knock down specific protein expression?

a. Synthetic siRNA

b. FRET

c. DNA expressing shRNA

d. Both A & C

8. The detection of specific proteins can be visualized by _____ by employing specific antibodies associated with a heavy metal marker, such as gold particles.

a. Cryo-EM

b. TEM

c. SEM

d. Metal shadowing

9. Which of the following is true?

a. TEM can be used on living samples

b. SEM can be used on living samples

c. Both TEM and SEM can be used on living samples

d. None of the above

10. Which of these anchors is used for proteins that are attached to the plasma membrane on the extracellular side?

a. Acylation

b. Prenylation

c. GPI anchor

d. Glycoproteins