Reference no: EM133814788
Question
TRIS (Tris(hydroxymethyl)aminomethane, also known as tris, Trizma, THAM, etc.) is commonly used in biochemistry as a buffering agent. Below is a graph showing the titration of a solution containing tris buffer titrated with a solution of NaOH Please note: The graph is zoomed in to a pH range of 4-13 so that you can clearly see the titration curve. The graph illustrates the pH change during the titration of a Tris buffer solution with hydrochloric acid (HCl), titrated using 0.02 M sodium hydroxide (NaOH). The x-axis represents the volume of NaOH added in milliliters (mL), ranging from 0 to 30 mL, while the y-axis measures the pH from 4 to 12. Initially, the pH starts around 5.5 and rises gradually as more NaOH is added, showing a smoother increase after around 15 mL, reflecting the buffering capacity of the Tris solution to resist changes in pH. This gradual increase continues until the pH levels off around 11.5, indicating the buffer's capacity has been surpassed. The curve demonstrates the typical sigmoidal shape expected in a buffer titration curve, useful for understanding the buffering behavior of Tris in biochemical applications.
1. Is TRIS monoprotic, diprotic, or triprotic?
2. At what volume (mL) is the equivalence point for this titration?
3. Based on the graph, what is the estimated pKa for TRIS? Estimate to the nearest 0.1 pH unit.
4. What is the effective buffering range for TRIS? Estimate to the nearest 0.1 pH unit.
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