Reference no: EM132792710 , Length: 2149 words

BHS002-1 Microbiology and Biochemistry Assignment - University of Bedfordshire, UK

Assessment - Practical Portfolio (Biochemistry)

Unit learning outcomes -

1. Present, analyse and interpret experimental data and clearly communicate your findings in writing.

2. Demonstrate understanding of the molecular and cellular mechanisms involved in metabolic pathways through the relationship between biochemical and biotechnological systems.

Required to do in this assignment - You will carry out two practicals:

1. In the first session you will carry out the Trinder's assay of Glucose. This will require calibration using a standard absorbance curve; identification of an unknown glucose concentration will follow.

2. In the second session you will use thin layer chromatography to analyse the behaviour of lipid samples.

Each lab report should contain four sections: Introduction, Results, Discussion and References.

Introduction: This section should typically represent approximately 30% of each report. It should summarise the published background literature relevant to this study. It must explain what your experimental study is about, and place it in context of the previously published literature. It should state the scientific aim of the study.

Results and discussion: This section should typically represent the remaining 60-70% of each report. Data may be presented in tables, graphs, diagrams, or photographs as appropriate for your particular experimental study. Figures and tables should be separately numbered, and be clearly labelled. You should include written text to explain what your findings are and what is shown in the figures and tables. Results should describe your findings/observations, but not interpret their meaning. In the discussion you should interpret your results, explaining what they indicate. You should evaluate the quality of your data. You should identify any problems with the technique or data (if any exist) and suggest possible solutions. You should compare your findings to previously published findings or your expected findings, and should place your results in the context of published scientific literature. Your discussion should also include a reflection on your performance within the practical. What insights did you gain from the practical sessions; what problems did you have to overcome etc.

References: You should include at least three peer-reviewed scientific journal articles or textbooks as sources. These should be listed in correct UoB Harvard format in a single reference list. The reference list should only contain sources that have been cited appropriately - eg (Smith, 2010) - in the main text of your report.

Special instructions -

Practical 1 - This should include tables showing all data obtained during the experiment in addition to graphs (produced using Excel or other graphing software) of the standard curve and a histogram for the second set of data. The handwritten data sheet from the experiment should be included as an appendix.

Practical 2 - Your write-up should include a data table similar to the one in the protocol. The signed off sketch of the tlc plate and the table of predicted solubilities/migration orders should form an appendix.

Attachment:- Microbiology and Biochemistry Assignment File.rar