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You have isolated a strain of mutant yeast cells that divides normally at 30°C but cannot enter M phase at 37°C. You have isolated its mitotic cyclin and mitotic Cdk and find that both proteins are produced and can form a normal M-Cdk complex at both temperatures. Which of the following temperature-sensitive mutations could not be responsible for the behavior of this strain of yeast? Explain your reasoning.
(a) inactivation of a protein kinase that acts on the mitotic Cdk kinase
(b) inactivation of an enzyme that ubiquitylates M cyclin
(c) inactivation of a phosphatase that acts on the mitotic Cdk kinase
(d) a decrease in the levels of a transcriptional regulator required for producing sufficient amounts of M cyclin.
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