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Basic steps to clone a gene into a vector for expression: (1)PCRamplificationofthe gene, (2) digest (cut) both the double-stranded PCR product (insert) and plasmid vector with the same restriction enzyme(s) to create complementary "sticky ends", (3) allow the digested insert and vector to anneal, and (4) ligate the annealed fragments together with DNA Ligase.
A. What additional step is required to generate a DNA template for PCR from a eukaryotic organism rather than a prokaryotic organism, and why?
B. Why is it necessary to re-circularize (ligate) a plasmid prior to transformation into cells?
A brass vat is 20 m long and room temperature 20 degree Celsius. How much longer is it when it contains boiling water at 1 atm pressure?
When you cook your scrambled eggs in the morning the heatdenatures the protein albumin; while cooking you would most likelyNOT be breaking the protein's
1. For what specific purpose was gram staining developed for? What is it used for today? 2. Why is the presence of air bubbles a sign of contamination? 3. Why is a gram stain helpful when monitoring bacterial contamination in food or investigating a ..
For many generations, the following genotypic frequencies were observed in a large population of dinosaurs: 4 percent AA, 32 percent Aa, and 64 percent aa.
Substrate-level phosphorylation occurs within the metabolic pathway where sufficient energy is released by given chemical reaction to drive the synthesis of ATP from ADP and phosphate. In which the metabolic pathways are substrate-level phosphorylati..
At a minimum, this type of laboratory facility is necessary to handle organisms that typically do not cause disease in healthy individuals and present a minimal
Bacterial sRNA's activate and repress translation by exposing or covering RBS (ribosome binding site) on mRNA. Riboswitches also activate and repress translation by exposing or covering RBS on mRNA. How do these two regulatory RNA's differ from ea..
1-as we continue our exploration of signaling pathways in cancer one important class of players not discussed in detail
Differentiate between eumetazoans and sponges and Describe the features of eumetazoans with radial symmetry.
Discuss the concept of homeostasis, and be sure to point out how this is maintained in the body and the inter-relatedness of all the organ systems.
What is your understanding of the term "Chemotherapy"? Has that definition broadened now? How or how not
What are embryonic stem cells (ES)? How do embryonic stem Cells differ from Adult stem cells?
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