User Account

All Pages

Basic steps to clone a gene into a vector for expression

Assignment Help Biology
Reference no: EM131995129

Basic steps to clone a gene into a vector for expression: (1)PCRamplificationofthe gene, (2) digest (cut) both the double-stranded PCR product (insert) and plasmid vector with the same restriction enzyme(s) to create complementary "sticky ends", (3) allow the digested insert and vector to anneal, and (4) ligate the annealed fragments together with DNA Ligase.

A. What additional step is required to generate a DNA template for PCR from a eukaryotic organism rather than a prokaryotic organism, and why?

B. Why is it necessary to re-circularize (ligate) a plasmid prior to transformation into cells?

Reference no: EM131995129

Questions Cloud

Light-independent reactions of photosynthesis : Distinguish between the light-dependent and light-independent reactions of photosynthesis. What does each require and produce?
Is there a correlation to direct airframe maintenance cost : Is there a correlation to direct airframe maintenance cost and whether there was a relationship between fleet age and direct engine maintenance cost?
Application for the use of cell and molecular biology : In at least one paragraph please help mwe to explain at least three application for the use of Cell and Molecular Biology and its benefits of microbiology?
What sorts of laws and regulations : In your opinion, what sorts of laws and regulations, if any, should society put into place to regulate the technology that Esvelt regrets championing?
Basic steps to clone a gene into a vector for expression : Basic steps to clone a gene into a vector for expression: (1)PCRamplificationofthe gene, (2) digest (cut) both the double-stranded PCR product (insert).
Is it possible to get genetic tests of other animals : Is it possible to get genetic tests of other animals? Why would you want to? How can karyotypes help in the study of evolution?
Why is it important for us to know where genes are located : Why is it important for us to know where genes are located? How does one use a linkage map? What advancements has the HGP led to?
Genetic material or the gain of genetic material : Explain how nondisjunction can result in loss of genetic material or the gain of genetic material.
Sperm of a man cannot swim : What cellular organelle or structure is affected when the sperm of a man cannot swim thus rendering him infertile.

Reviews

Write a Review

Biology Questions & Answers

  Long and room temperature

A brass vat is 20 m long and room temperature 20 degree Celsius. How much longer is it when it contains boiling water at 1 atm pressure?

  Scrambled eggs in the morning the heatdenatures the protein

When you cook your scrambled eggs in the morning the heatdenatures the protein albumin; while cooking you would most likelyNOT be breaking the protein's

  Investigating a foodborne illness outbreak

1. For what specific purpose was gram staining developed for? What is it used for today? 2. Why is the presence of air bubbles a sign of contamination? 3. Why is a gram stain helpful when monitoring bacterial contamination in food or investigating a ..

  What percentage of these newborn dinosaurs died

For many generations, the following genotypic frequencies were observed in a large population of dinosaurs: 4 percent AA, 32 percent Aa, and 64 percent aa.

  Create a controlled experiment to test hypothesis

Substrate-level phosphorylation occurs within the metabolic pathway where sufficient energy is released by given chemical reaction to drive the synthesis of ATP from ADP and phosphate. In which the metabolic pathways are substrate-level phosphorylati..

  Minimal threat to the environment and lab personnel

At a minimum, this type of laboratory facility is necessary to handle organisms that typically do not cause disease in healthy individuals and present a minimal

  Repress translation by exposing or covering rbs on mrna

Bacterial sRNA's activate and repress translation by exposing or covering RBS (ribosome binding site) on mRNA. Riboswitches also activate and repress translation by exposing or covering RBS on mRNA. How do these two regulatory RNA's differ from ea..

  1-as we continue our exploration of signaling pathways in

1-as we continue our exploration of signaling pathways in cancer one important class of players not discussed in detail

  Differentiate between eumetazoans and sponges

Differentiate between eumetazoans and sponges and Describe the features of eumetazoans with radial symmetry.

  Discuss the concept of homeostasis

Discuss the concept of homeostasis, and be sure to point out how this is maintained in the body and the inter-relatedness of all the organ systems.

  What is your understanding of the term chemotherapy

What is your understanding of the term "Chemotherapy"? Has that definition broadened now? How or how not

  What are embryonic stem cells

What are embryonic stem cells (ES)? How do embryonic stem Cells differ from Adult stem cells?

Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

Submit Assignment

Academics

Major Subjects

Majors

Get In Touch

whatsapp: +1-415-670-9521

Phone: +1-415-670-9521

Email: [email protected]

TERMS & POLICIES

HELP & SUPPORT

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd