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Basic steps to clone a gene into a vector for expression: (1)PCRamplificationofthe gene, (2) digest (cut) both the double-stranded PCR product (insert) and plasmid vector with the same restriction enzyme(s) to create complementary "sticky ends", (3) allow the digested insert and vector to anneal, and (4) ligate the annealed fragments together with DNA Ligase.
A. What additional step is required to generate a DNA template for PCR from a eukaryotic organism rather than a prokaryotic organism, and why?
B. Why is it necessary to re-circularize (ligate) a plasmid prior to transformation into cells?
Why is there are distinct differential diagnosis for neonatal and non-neonatal conjunctivitis.
What is a microbial colony.
Would this be a reduction of cancer risk, correction of cancer gene or destruction of cancerous tissue?
The assignment is to find a recent biological or medical discovery/innovation, and write a two-page summary that includes (1) general background on the field of biology or medicine.
A horse heterozygous for these two alleles is a palomino (golden body color with flaxen mane and tail). What exception to Mendel does this demonstrate
q1. choose two products from your daily diet as in butter or salad dressing and find similar products that use fat
Assume that, during biological research, it became possible to incorporate fully functional chloroplasts into the basal layer of our epidermal skin cells. Knowing the structure and function of chloroplasts, how will this influence our body.
q1. michael lynch and john conery showed that there is a strong negative correlation between nemu and genome size. how
Which of the following factors are decrease the metabolic rate and The process that form glucose from noncarbohydrate sources is
Draw a Punnett Square crossing BbRr x bbrr knowing that the genes are loosely linked. Crossing over can occur, butat a relatively low rate.
A male and a female are each heterozygous for both cystic fibrosis (CF) and phenylketonuria (PKU). Both conditions are autosomal recessive, and they assort independently.
Discuss when flavoprotein transfers electrons directly to final electron acceptor, hydrogen peroxide is produced. What other consequences might result from electron carriers in the ETC being bypassed?
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