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Here's one experimental approach to finding out how rapidly a membrane transporter can move its solute into the cell: combine biochemical information about how much solute accumulates in the cell over time with structural information about how many transporters are on the surface of the cell. You are studying a spherical eukaryotic cell that has a diameter of 17 ?m and is engaged in glycolysis. Its energy source is glucose, which it is taking up from its environment via Na/glucose symporters distributed throughout the surface of its plasma membrane. By briefly adding an inhibitor of all glucose breakdown, you are able to determine that glucose accumulates in the cell at the rate of 47 ?M/hr.
Based on this number, how many ?moles of glucose must be entering the cell per hour?
(Assume that one-half of the volume of the cell is composed of organelles, and thus not available for diffusion of glucose)
What is the flux of glucose into the cells (flux is expressed in moles per cm2 per sec)?
If electron microscopy indicates that there is on average 1 Na/glucose symporter per ?m2 of the plasma membrane, at what rate must each symporter transport glucose, in molecules per second?
One blood glucose meter always reads 10 too high. Another meter reads anywhere from 5 to 9 too high. Which one is the more precise? which one is the more accurate? How is accuracy different from precision?
The information presented in Problem seven are analyzed using multiple linear regression analysis and the models are shown here. In the models, the data are coded as 1= new.
Just before a physiology exam her ventilation increases to 18 breaths per minute from nervousness. Now what is her total pulmonary ventilation? Assuming her anatomic dead space is 120 mL, what is her alveolar ventilation in each case?
Dynein is missing from cilia and flagella of the individual with a specific inherited disorder. These individuals have severe respiratory problems, and if males, are sterile. What is the structural relationship between these 2 symptoms.
Carbon fixation requires the expenditure of ATP molecules which is generated by A. formation of glucose during the Calvin cycle B. replenishment of chlorophyll C. ETS.
Fluorescently labeled annexin V is mixed with each vesicle preparation. The vesicles are washed to remove unbound annexin V and next examined by fluorescence microscopy. Presence of annexin V is detected by green fluorescence, as shown above. What ca..
You have discovered the gene that encodes an enzyme to dissolve fat. You would like to produce large quantities of this enzyme and harvest it from bacteria. You have isolated the gene and introduced it into bacteria. What problems might you encoun..
The trait for short, stiff hair is recessive to long, furry hair, & purple color is recessive to black color. A purple furry female is crossed to black short hair male.
Determine a news report of a current outbreak or other health crisis (within a month) then search for a peer-reviewed scientific article on the same or closely related topic
A prison guard states that an inmate threw and unknown liquid on him. This is a felony charge of harassment. In order for this charge to be prosecuted, you must show a bodily fluid was thrown on the guard.
Cutting DNA with restriction enzyme, hybridizing the DNA with probe, electrophoresis of DNA, blotting the DNA.define the right order in which procedures are carried out.
Many bacteria are anaerobic. If bacterium were to produce the same amount of ATP within a minute as a cell extracted from your skeletal muscle, what would that bacterium have to do?
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