Assure the preparation of a truly pure culture

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You have a microorganism you know is both fastidious and which normally lives in human lungs. Which medium might this organism have the highest probability of growing on and why?

If you were interested in isolating a wild microorganism in pure culture, one which you suspect makes up approximately half of the cells in your crude mixture, name a technique (other than the use of selective media or enrichment culture) that you would have to employ in order to accomplish this isolation, and tell me a key aspect of how you would have to use it in order to assure the preparation of a truly pure culture.

Reference no: EM132695475

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