Reference no: EM132855490 , Length: 1310 words
Assignment - Molecular Pathology Laboratory Management
Contents of a standard test procedure (an example of a PCR-RFLP test)
1. Background - Theoretical information concerning the disorder and the test.
2. Principle of the assay - Description of how the restriction enzyme detects the specific mutation.
3. Summary of the assay - Characteristics of the restriction-based assay used.
4. Clinical significance - Use of the test for patient care.
5. Strategy for analysis - Overview of the testing steps (i.e., extraction. PCR amplification, digestion, gel electrophoresis).
6. Specimen collection or acquisition - Description of acceptable specimen type, storage, shipment, and required clinical information.
7. Criteria for rejected samples - Description of unacceptable samples (i.e., specific specimen types, improper labeling, damage).
8. Equipment and supplies - List of equipment necessary for testing (i.e., PCR machine, gel electrophoresis).
9. Reagents - List of reagents used for each test step (e.g., PCR, restriction enzyme digestion, gel electrophoresis equipment).
10. Assay procedure - Description of the testing steps, including general considerations, sample preparation, pre-PCR preparation and setup, thermal cycling, PCR cleanup, PCR product digestion, gel preparation and electrophoresis, gel documentation.
11. Analysis of results - Description of expected results, control results, criteria for gel analysis and interpretation, documentation of results in the LIS and laboratory database, if used.
12. Turnaround time and schedule for retaining specimen - Expected turnaround time for the specific test, specimen and DNA storage, and schedule for retaining the specimen and DNA.
13 References - Literature or commercial-based information.
14. Addenda - E.g., the worksheet for the specific test, the manufacturer's product insert.
Attachment:- Lab Report Guide File.rar