Assess the quality of the purified protein

Assignment Help Biology
Reference no: EM132934052

Q1. Evaluate the scientific evidence that longevity is genetically controlled in both model organisms and humans, and critically discuss whether current evidence supports the idea that the extension of human longevity is possible, for example by pharmacological or life-style intervention.

Q2.-You want to express and purify an intracellular 30 kDa soluble protein that has a theoretical pI 5.5. The protein also has an engineered His-tag, which can be cleaved by TEV protease. There are also antibodies available for the protein to be purified.

Critically evaluate the choices you have in expression systems and purification strategies to produce a pure protein sample suitable for protein crystallography. Include in your answer, a description of how you would assess the quality of the purified protein throughout the process

Reference no: EM132934052

Questions Cloud

What is the beta for security j : If the risk-free rate is 4.0%, the expected return on the market is 17.0%, and the expected return on Security J is 19.1%, what is the beta for Security J?
Observing bacteriophage dna changes : Werner Arber was the first to hypothesize how these enzymes work by observing bacteriophage DNA changes.
What is value of after-tax cash flow associated with sale : You are evaluating a potential investment in equipment. What is the value of the after-tax cash flow associated with the sale of the equipment?
Draw a rich picture of the identified problem : Draw a Rich Picture of the identified problem/challenge/issue in MGT603 Assessment 1 (Part A). The Rich Picture can be hand drawn and should provide a concise
Assess the quality of the purified protein : Critically evaluate the choices you have in expression systems and purification strategies to produce a pure protein sample suitable for protein crystallography
What is the after-tax operating cash flow for year four : Trucks is $135,000 per year with annual operating costs of $69,000. The firm's marginal tax rate is 25.0%. What is the after-tax operating cash flow for year 4?
What is the after-tax operating cash flow for year two : What is the after-tax operating cash flow for year 2? The equipment is expected to generate revenues of $115,000 per year with annual operating costs of $61,000
Determine the internal rate of return for a project : Determine the internal rate of return for a project that costs -$156,000 and would yield after-tax cash flows of $24,000 the first year, $26,000 the second year
Determine the net present value for a project : Determine the net present value for a project that costs $227,000 and would yield after-tax cash flows of $24,000 per year for the first 7 years

Reviews

Write a Review

Biology Questions & Answers

  T cell responses are generated to fight infections.

T cell responses are generated to fight infections.

  Use the eyeless mrna as a probe

Aniridia is a human condition in which the eye has no iris. The protein encoded by the gene responsible for Aniridia is very nearly identical to the protein product of the fly eyeless protein.

  Explain how does the misuse of antibiotics influence the

q1. why is the russian prison system considered to be ground zero in the fight against tb? q2. what is responsible for

  How many chromosomes will the daughter cells have

In which stage of mitosis are the chromosomes lined up on the cell's equator? If a parent cell has 46 chromosomes prior to mitosis, how many chromosomes will the daughter cells have?

  Why enhancers can be moved around within the dna

How do you explain why enhancers can be moved around within the DNA without affecting their activity, whereas the TATA box can only operate at one specific site?

  Determine firm new required rate of return

Consider the information below for Postman Builders Inc. Suppose that the expected inflation rate and thus the inflation premium increase by 2.0 percentage poin

  Describe the causative agent of the potential diagnosis

Low-grade temperature, thrush in the oral cavity, fatigue, weakness, rash on trunk and legs - What tests would you recommend? Choose one of the tests and describe the causative agent of the potential diagnosis.

  What corrective action to take in-flight

With that said, describe what a "black hole" illusion is, what corrective action to take in-flight, and how an aviator can prevent it from occurring.

  Genotype with a homozygous recessive individual

A testcross is performed to figure out the unknown genotype of an individual. Take for example a black rabbit. The fur color black is dominant over the white

  Aspirin is a weak acid

Aspirin is a weak acid (see figure below) that is taken up into the bloodstream by diffusion through cells lining the stomach and the small intestine. Aspirin crosses the plasma membrane of a cell most effectively in its uncharged form; in its charge..

  Responsible for global warming

Carbon dioxide is a greenhouse gas that is thought to be responsible for global warming. Using this information, explain why destruction of the Earth's rain

  Congenital disorders of the skeleton system

Discuss the anatomy of the pathology and how it changes the organ system.  In other words, what is the pathology and how does it change the functions of the organ system you selected!

Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd