Assess the quality of the purified protein

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Q1. Evaluate the scientific evidence that longevity is genetically controlled in both model organisms and humans, and critically discuss whether current evidence supports the idea that the extension of human longevity is possible, for example by pharmacological or life-style intervention.

Q2.-You want to express and purify an intracellular 30 kDa soluble protein that has a theoretical pI 5.5. The protein also has an engineered His-tag, which can be cleaved by TEV protease. There are also antibodies available for the protein to be purified.

Critically evaluate the choices you have in expression systems and purification strategies to produce a pure protein sample suitable for protein crystallography. Include in your answer, a description of how you would assess the quality of the purified protein throughout the process

Reference no: EM132934052

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