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DNA replication in early Drosophila embryos occurs about every 5 minutes. The Drosophila genome contains approximately 1.8 X 108 base pairs. Eukaryotic DNA polymerases synthesize DNA at a rate of approximately 40 nucleotides per second. Approximately how many origins of replication are required for this rate or replication? (Hint: remember that replication is bi-directional.)
Explain the transport properties of the loop of Henle and describe the interactions between the ascending and descending limbs in the countercurrent multiplier system
Describe the developmental program of a T cell, including positive and negative selection and changes in phenotypic markers
Reach capabilities in the sagittal plane can be determined graphically using anthropometric data as shown in the figure on the given page.
What is a chloride reversal potential. Is there a net movement of chloride into or out of the cell while you add GABA to the bath? If so, in which direction.
Assume that you measure the OD of two E.coli cultures: culture A and culture B. The spectrophotometer gives you a reading of 3.2 for culture A and 0.01 for culture B. What would you do to determine the concentration of culture A and B and why? Giv..
Phenylketonuria is extremely common in Ireland, affecting approximately 1 in 4500 live births. Determine the frequency of PKU carriers?
define conduction velocity.
Hermann Kolbe's synthesis of an organic compound, acetic acid, from inorganic substances that had been prepared straight from pure elements was an important milestone for what reason.What piece of evidence contradicts this claim.
Discuss the difference between transformation and electroporationwhen? when do we use transformation and when do we use electroporation?
Calculate the maximum [glucose]in/[glucose]out ratio that can be achieved by the plasma membrane Na+-Glucose symporter of an epithelial cell when the concentration of Na+ inside the cell is 12 mM.
Your assistant Igor is studying a neurotransmitter-stimulated GPCR pathway, hypothesized to stimulate the expression of genes that increase retention of memory.
Fluorescently labeled annexin V is mixed with each vesicle preparation. The vesicles are washed to remove unbound annexin V and next examined by fluorescence microscopy. Presence of annexin V is detected by green fluorescence, as shown above. What ca..
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