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You need to calculate ligation for your insert. You know that you have 3 ng/μL in your sample, how many picomols/μL do you have if it is a 1000 bp insert?
Include your converstions and calculations.
Tips:A base pair has an approximate molecular mass of 660 g/mol*bp.
What similarities do you see in the recognition/cleavage sequence for these restriction enzymes?Hhal GCGC, Hinfl GANTC, Alnl AGCT and Dral TTTAAA. What doesthis say about how restriction enzymes recognize their sites?
The replication cycles of bacteriophages and animal viruses have many similarities. Discuss similarities and differences of the characteristics of prophages vs proviruses.
Describe three ways that B cells and T cells are similar and at least five major ways in which they are different. 100 words minimum and references.
amount of water displaced by potato samples before and after experiment potato typepotato a beginning displacement
Where did drwin spend most of his life studying animals andevolution? What was Darwin's ship's name?
Your instructor asks you to isolate and identify the organisms in an unknown culture. You find that the culture contains two gram-negative bacilli that produce swarming colonies. What biochemical test would you use to identify the bacilli?
Which one of the following statements is true.
A fruit fly population has a gene with two alleles, A1 and A2. Tests show that 70% of the gametes produced in the population contains the A1 allele. If the population is in Hardy-Weinberg equilibrium, what proportion of the flies carry both A1 and A2..
There are four characteristics to a healthful diet. Please list these characteristics and provide an explanation for them as well.
In the fungus Neurospora, the td+ allele is needed for the cells to build the amino acid tryptophan. The mutant allele td results in an inability of the fungus to make its own trp,
What differences in the antibodies produced following immunization with the inactivated virus contained int he " Flu Shot" vs. the attenuated virus contained in the " flu mist" vaccines do you expect? Which would you recommend for individuals who ..
In the last stage of cellular respiration, oxidative phosphorylation, all of decreased electron carriers produced in the previous stages are oxidized by oxygen via the electron transport chain.
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