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Q. Suppose a mammalian genome (4x10^9bp in size) is digested with Hind III, and you are cloning a unique fragment of 2kb.
A. Approximate how numerous clones you will need to screen?
B. If you first size select the genomic DNA, realizing a 10-fold purification, and clone that DNA in a plasmid, now how many clones will you need to screen?
C. If you transform bacteria with the plasmid library made in part b above, divide the transformatnts into 20 aliquots and find one has the insert of interest, now how many clones do you need to screen?
D. If you screen 5,000 colonies on a 15cm dish, how various dishes will you require to find a clone of interest? "
Some wines are marketed as "sparkling" wines. Sparkling wines are considered so because of a dissolved gas that makes them fizzy. Concern what you know about the procedure of respiration to explain the type and origin of this gas.
Suppose that P is always completely dominant to any number of p's, and that the locus is close to the centromere. What is an expected ratio of purple:white in the progeny of the next crosses.
Which of the follow was necessary to this confirming experiment. Phosphorus is not present in amino acids in the protein coat of phage.
What are the disadvantage and advantage of each and which commonly applies to hospitality industry. How is this caused by the sympathetic nervous division.
Trace a route of air from the nares to an alveolus. Name subdivisions of organs where applicable, and discriminate between conducting and respiratory zone structures. how large a force does she exert on the sled.
Illustrate one experiment that shows that shh is the critical molecule produced by the inducing tissue referred to in (a). what is the evidence that this tissue regulates the ventral pattern of the neural tube (even before this tissue was known to pr..
Black coat of animal is dominant; white is the alternative recessive. While true-breeding black is crossed to true-breeding white, what is the possibility that a black F2 is heterozygous.
What is the possibility that their next child will show both defects? Demonstrate the work.
Find out both the allelic frequencies and the genotypic frequencies. A 68-year-old client was admitted to the hospital medical floor with a diagnosis of chronic bronchitis. His wife asks the nurse why his skin looks blue. How would you describe cyano..
What percent of the population are homozygous dominant. What do you assume would be the effect on the switch of reversing orientation of one of the hix sites.
Distinguish between mechanical advantages and disadvantages in lever systems and find out which kind of lever the doctor was using to test quadriceps muscle.
The client is 52years old and has a history of hypertension. His cholesterol level is 245. He states his job is very stressful. He admits he is overweight. His father died of stroke at age 60. The nurse encourages him to eat fish. Why is the nurse te..
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