Reference no: EM132523241

You have a sample of milk and you start making dilutions by adding 1 ml of it to 9 ml of water in (tube #1) and then add 1 ml aliquot from tube #1 to 9 ml of water to next tube (tube #2) and the you add a 1 ml aliquot from tube #2 to 9 ml of water in next tube (tube #3) and your repeat this same dilution pattern for 2 more tubes ( #4 and #5).

You take samples of 0.1 ml from each of the 5 tubes and spread it on surface of a nutrient agar plate. After 48 hours, you count the number of colonies on each agar plate and you get these results.

Tube # Dilution Factors # of colonies
1 TNTC*
2 TNTC
3 500
4 50
5 TFTC*

*TNTC (too numerous to count)
*TFTC (too few to count)

Indicate the dilution factor of tubes 1 through 5 in the space provide above in 2 different formats for example 1:10 will be the same as 10-1.

From the numbers of colonies shown in table above, use the most cin your original culture.

5. You need to make serial dilution of a cell suspension and you start at 1:10 dilution and need to go all the way to 10-6 dilution. How many test tubes you need to make up all your dilutions?

6. You have a 10 ml vial of penicillin with concentration of 2 mg/ml. You are asked to inject 0.5 ml of that to your patient. How many micrograms of the drug your patient is receiving?

7. You have a suspension of Vancomycin at concentration of 4 mg per ml. You are asked to inject your patient with 10 g of drug per kilogram of the body weight. If your patient's weight is 100 kg, what volume of drug you would inject to your patient?