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Applying the michaelis-menten equation

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Applying the Michaelis-Menten Equation I

A research group discovers a new version of happyase, which they call happyase*, that catalyzes the chemical reaction HAPPY to SAD The researchers begin to characterize the enzyme.

(a) In the first experiment, with [E] at 4 nM, they find that the Vmax is 1.6u M s-1. Based on this experiment, what is the kcat for happyase*? (Include appropriate units.).

(b) In another experiment, with [E] at 1 nM and [HAPPY] at 30 uM, the researchers find that V 300 nM s-1. What is the measured Km of happyase* for its substrate HAPPY? (Include appropriate units.).

Reference no: EM131740746

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