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Q. Two membrane preparations have been derived from plasma membrane of erythrocytes and are present in a buffer solution:
(1) right-side-out membrane vesicles, in which exoplasmic leaflet of the vesicle faces the buffer (equivalent in orientation to that of the exoplasmic leaflet of the intact plasma membrane); and
(2) inside-out membrane vesicles in which the exoplasmic leaflet faces the interior of vesicles.
Annexin V is a protein that binds to phosphatidylserine, one of the phospholipids present in cell membranes.
Fluorescently labeled annexin V is mixed with each vesicle preparation. The vesicles are washed to remove unbound annexin V and next examined by fluorescence microscopy. Presence of annexin V is detected by green fluorescence, as shown above. What can be deduced from these data?
What is the total ocean area that (trillion m2) that can be classified as open ocean, that is, ocean that is greater than 960 km from the next land.
The client tells nurse that he does not understand what that means. How would nurse explain this in terms the client would understand.
The most significant probable cause of this temporary apnea is decreased activity of the what. what is the probability that the next child would be a girl.
Plants lacking this enzyme grow slowly and are often light green or white in colour. Provide explanations with support for both these plants' slow growth rates and light-green or white appearance.
When an experiment is repeated with a protein medium, yeasts grow only in the presence of oxygen. Pasteur concluded that the yeasts can take oxygen from air, and in the absence of air, the yeasts take oxygen from sugar.
Medical history elicits the facts that she lost her husband six months previously, has been extremely depressed, and is taking sleeping pills and not eating properly. What would doctor hypothesize about the probability of senile dementia in this case..
ATP-synthase is a large membrane-integral protein complex that produces ATP from ADP and inorganic phosphate. Describe the location of this complex in the cell; list its major parts and the subunits each part is made of. Describe how it is able to co..
What percent of all the enzyme molecules will have their Cys thiol in the active form at pH 8.6. Some mammals might run or fight when frightened. For both of these examples, illustrate the physiological mechanisms involved in the answer.
The both endocrine and the nerve system are major regulating system of the body; however, the nervous system has been compared to an airmail system and the endoncrine system to the pony express. What is the resemblance.
A subject extends his arm palm up to catch a falling object. The contact of object with the hand triggers a reflexive response in the biceps muscle. But this response is different and opposite if the object is a raw egg instead of a ball. Explain why..
The supernatant from normal cells corrects the defects in B and C, but not defect in A. Hurler's disease is as a result of the failure to make one particular lysosomal enzyme. The supernatant from A corrects the defect in Hurler's cells, but supernat..
Acetylcholine binds this membrane protein, which subsequently opens to allow sodium to enter the cell. Acetylcholine exerts its effect on what kind of transport protein.
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