Analyze a sample of dna, To be able to properly analyze a

Analyze a sample of dna

Reference no: EM1395460

To be able to properly analyze a sample of DNA, you need to have at least 1 ug of the DNA of interest. Assumptions: PCR is run with 30 cycles; Molecular weight of nucleotide = 330 g per mole for a single stranded DNA.

a. Given that you can use the Polymerase Chain Reaction (PCR) to amplify DNA from the original sample and the size of the fragment to be amplified is 10,000 nucleotides long, estimate how many DNA molecules you need to obtain the minimum amount required to analyze the sample. HINT: X = X0*2n , where X is the amount (or number of molecules) of DNA produced after 'n' cycles and X0 is the starting amount (or number of molecules) of DNA prior to initiation of cycles.

b. Suggest a way to increase your sensitivity, i.e. amplify the DNA starting from even fewer molecules of template DNA.

Reference no: EM1395460

Questions Cloud

Question about parkinson disease : PC12 cells produce dopamine and so they may be useful in treating Parkinson's disease (when dopamine is missing). One approach would be to encapsulate PC12 cells inside a spherical capsule.

How liquid and solid forms of media are prepared : Know how liquid (broth) and solid forms of media (slants, deeps, petri dishes) are prepared and sterilized in an autoclave.

Finding confidence interval of the true mean : The winning team's score in 13 high school basketball games was recorded. If the sample mean is 54.3 points and the sample standard deviation is 11.0 points, find the 98% confidence interval of the true mean.

Sign of correlation coefficient- sign of slope of regression : What is the relationship between the sign of the correlation coefficient and the sign of the slope of the regression line?

Analyze a sample of dna : To be able to properly analyze a sample of DNA, you need to have at least 1 ug of the DNA of interest. Assumptions: PCR is run with 30 cycles; Molecular weight of nucleotide = 330 g per mole for a single stranded DNA.

Predicted values of response variable : On a graph, the vertical difference between the observed and predicted values of the response variable for an individual is called the

Binding of oxygen to hemoglobin : When a person exercises, muscle tissue produces both fixed acids (lactic acid) and volatile acids (CO2). In terms of the binding of oxygen to hemoglobin, how might this mild acidosis be beneficial to the person?

Women in workforce : If you were to draw a scatter plot of the number of women in the workforce versus the number of Christmas trees sold in the United States for each year between 1930 and the present

Pairs of points yielded a correlation coefficient : Ten pairs of points yielded a correlation coefficient y of 0.79. If a=0.05, which of the following statements is correct if H 0:P=0? (Do not calculate a t-value.)

User Account

All Pages