Amino acid sequence from the data

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Reference no: EM13775649

1. Determine the amino acid sequence from the following data.

Composition: 3 Ala, Arg, Asp, Gly, His, Leu, 2 Lys, 2 Met, Thr, Trp, Tyr, Val

Edman: Ala-

Tryptic peptides:

Tp-1: Ala, Asp, Lys, Trp

Edman: Ala-

Tp-2: Ala, Arg, Gly, Met, Thr

Edman: na

Tp-3: Ala, His, Leu, Lys, Met, Tyr, Val

Edman: na

Chymotryptic peptides:

Ch-1: Ala, Asp, Trp

Edman: Ala-

Ch-2: His, Leu, Lys, Met, Tyr, Val

Edman: na

Ch-3: 2 Ala, Arg, Gly, Lys, Met, Thr

Edman: na

CNBr peptides:

C-1: Ala, Arg, Gly

Edman: Ala-

C-2: Ala, Asp, His, Homoser lactone, Lys, Trp

Edman: Ala-

C-3: Ala, Homoser lactone, Leu, Lys, Thr, Tyr, Val

Edmn: Leu-

2. Determine the amino acid sequence from the data.

Composition: Ala, Arg, Asp, 2 Gly, Ile, 2 Leu, 2 Lys, Met, Phe, Ser, Thr, Tyr, Val

Edman on whole peptide: Gly-

Tryptic peptides:

Tp-1: Composition: Ala, Gly, Lys, Tyr

Edman Gly-

Tp-2: Composition: Arg, Gly, Leu, Ser, Thr

Edman: Gly-Thr-Leu-

Tp-3: Composition: Asp, Ile, Leu, Lys, Met, Phe, Val

Edman: Phe-

Chymotryptic peptides:

Ch-1: Composition: Gly, Tyr

Ch-2: Composition: Asp, Ile, Leu, Lys, Met, Val

Edman: Asp-

Ch-3: Composition: Ala, Arg, Gly, Leu, Lys, Phe, Ser, Thr

Edman: Ala-

CNBr peptides:

CNBr-1: Composition: Ile, Leu, Lys, Val

Edman: Val-Leu-

CNBr-2: Composition: Ala, Arg, Asp, 2 Gly, Leu, Lys, Homoser lactone, Phe, Ser, Thr, Tyr

Edman: Gly-Tyr-

Reference no: EM13775649

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