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1. We will verify that we purified GFP using western blotting with a specific GFP antibody. What other antibody could be have used to achieve the same conclusion?
2. Could we have used in-gel fluorescence to see if we have purified GFP? Describe what in-gel fluorescence is and what we would have had to do differently to use this technique.
3. How could we use the purified GFP to make a specific antibody against GFP?
4. Imagine that we need GFP of higher purity. Discuss ways we could improve the GFP purification. In your answer, explain the use of "TAP-tags".
5. GFP is a relatively easy protein to express in bacteria, as it is soluble and non-toxic, even at high expression proteins. Not all proteins are so easy to work with. Discuss the difficulties and strategies to overcome these difficulties when using bacteria to express:
i. proteins which are toxic to the bacteria
ii. membrane bound proteins
Is blood pressure purely determined by genetics? Why or why not? What four suggestions would you give someone to increase their blood flow? Why did you choose
Black coat color (B) in rats is dominant to brown coat color (b). There is a cross between a homozygous recessive and a heterozygote. What is the chance of getting an offspring with the homozygous recessive phenotype?
Under anaerobic conditions (a lack of oxygen), the conversion of pyruvate to acetyl CoA stops. Which of these statements is the correct explanation for this observation?
A congenital defect in the liver enzyme fructose 1-6 bisphosphate results in high level of lactate in the blood plasma.
What is the maximum increase in redox potential that can be induced by a 1000 nm photon?
Assume that a dihybrid F2 ratio, resulting from epistasis, was 9:3:4. If a double heterozygote was crossed with the fully recessive type, what phenotypic ratio is expected among the offspring?
Which of the following includes Highest levels of hierarchy?
Two ferredoxin molecules, carrying one electron each, transfer two electrons to NADP+, which picks up a proton and becomes NADPH.
Should the countries keep the virus or destroy the remaining vials of the virus? Explain your reasoning?
Readed reading. Epidemiological studies can evaluate risks of disease and protections from disease.
Describe cell death and how it contributes to morphogenesis. Include a comparison of programmed cell death to "accidental" cell death.
Please include detailed pharmacological mechanisms of how this occurred, and your subsequent steps in her management.
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