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1. We will verify that we purified GFP using western blotting with a specific GFP antibody. What other antibody could be have used to achieve the same conclusion?
2. Could we have used in-gel fluorescence to see if we have purified GFP? Describe what in-gel fluorescence is and what we would have had to do differently to use this technique.
3. How could we use the purified GFP to make a specific antibody against GFP?
4. Imagine that we need GFP of higher purity. Discuss ways we could improve the GFP purification. In your answer, explain the use of "TAP-tags".
5. GFP is a relatively easy protein to express in bacteria, as it is soluble and non-toxic, even at high expression proteins. Not all proteins are so easy to work with. Discuss the difficulties and strategies to overcome these difficulties when using bacteria to express:
i. proteins which are toxic to the bacteria
ii. membrane bound proteins
It is a very curcial concept to understand how the immune response is mounted against viruses, bacteria, protozoans and helminthes. For an effective immune response, both innate and adaptive immunity should work together.
This Project report elaborates a critical review of important elements attached to Advanced Glycated End Products (AGEs). It is very crucial to understand the process called Millard reaction.
Soil stabilization is the permanent physical and chemical alteration of soils to enhance their physical properties. Stabilization can increase the shear strength of a soil and control the shrink-swell properties.
This assignment has three parts which contains questions related to Microbiology. It contains basic principles of microscopy, staining techniques in microbiology and microbial growth in the food industry.
Lipid metabolites are often seen as key elements in cellular signaling. Is this unique? Please provide several examples of the function of lipids as key elements in signal arrays and list the biologic functions these signals affect?
Please describe how one might search for chemical structure, biologic function relationships, involving small molecular weight lipophylic compounds. Provide one example.
Write a case study which detailing a scenario of a patient being investigated in the Haematology laboratory.
The use of PCR and genetic approaches in biotechnology
Glucose oxidase is an enzyme that can be used for measurements of glucose levels by combining this reaction with an oxygen probe.
What phenotypic ratio would you get if you crossed a white mouse and a heterozygous brown mouse?
Prepare an essay on nosocomial infection.
To increase the awareness of monitoring and recording the blood pressure of patients and practice measuring blood pressure in a safe environment.
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