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You have just read a paper that the addition of caffeine to cells disrupts the DNA damage checkpoint and you would like to understand why. By searching for proteins that bind to caffeine, you isolate a protein, Caf1. You believe that Caf1 is involved in the DNA damage checkpoint because when you delete Caf1 from yeast cells and add a drug that causes DNA damage, the cells fail to arrest in the cell cycle. You give your Caf1 deleted cells to an undergraduate in the laboratory and ask him to take care of the strain for you while you take the weekend off to celebrate your finding. Upon your return, you find the poor undergraduate in tears. He explains that he thinks that he messed up the Caf1mutant strain while you were gone because after growing Caf1 and wild type cells in rich media and examining them during mitosis, Caf1 mutants looked the same as wild type cells. Are you concerned about the undergraduate student's findings? Why?
Explain the importance of the scientific method, sample size, controls and variable in the drug evaluation process as well as the dependent and independent variables for a drug that is proposed to control hypertension.
What environmental conditions would favor plant species with a strategy of producing many small seeds rather than a few large ones? What classification would we give to species utilizing one of these two strategies? Name and describe each.
Determine the pathophysiology for intrinsic vs extrinsic asthma? Are they both IgE mediated and Write out the overall chemical reaction of cellular respiration.
The cleavage of a peptide bond by chymotrypsin is caused by a nucleophilic attack by an active-site residue. Which of the following is true?
In this situation, you sampled gene pool without replacing beads in the beaker after you drew each one.
Wild-type fruit flies, D. melanogaster, have dark red eyes. Recessive mutations in the X-linked white gene result in white eyes.
lex A is an E. coli gene whose product regulates the transcription of certain target genes that are located at different positions in the bacterial chromosome.
Assume that a certain bacterial chromosome has one origin of replication. Under some conditions of rapid cell division, replication could start again from the orgin before the preceding replication cycle is complete.
Discuss what you would require to know to answer the following question: of all proteins that exist on earth at this time, what fraction of them have a known structure?
How does red bone marrow produce blood cells that function in the body's immune response?
Determine which of the following pairs of functional groups CANNOT react with each other by a dehydration reaction?
What is the relationship between rate of recombination anddistance between two genes?
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