Acid phosphatase was assayed at s 2x10-5 m the km for the

Reference no: EM13645282

Acid Phosphatase was assayed at [S] = 2x10-5 M. The Km for the substrate was 5x10-4 M. At the end of one minute 2.5% of the substrate, pNPP, had been converted to pNP (a product). Answer the following questions using this information:

a) What percent of pNPP will be converted at the end of 3.5 minutes assuming product formation is in 1st order of reaction?
b) What will be the product concentration at the end of 3.5 minutes?
c) Determine maximal velocity (Vmax) with the enzyme concentration used.

Reference no: EM13645282

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