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Absorbance measurements for the dye solutions

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Reference no: EM132410129

(1) EXPERIMENT 1: Record your absorbance measurements for the dye solutions in the table below.

Wavelength (nm) Absorbance Yellow 5 Dye Absorbance Red 40 Dye Absorbance Blue 1 Dye

400

420

440

460

480

500

520

540

560

580

600

620

640

660

680

700

(2) Record the absorbance values for the Red 40 and Blue 1 dye solutions in the table below.

Cuvette # Absorbance Red 40 Absorbance Blue 1

1

2

3

4

(3) What wavelengths did you use to measure the absorbance of the dyes in the orange drink?

(4) Using the equation for the lines in your calibration plots, calculate the concentration of the dyes present in your sample based on their absorbance.

(5) Given that the path length of the cuvette is 1 cm, what is the extinction coefficient of the 0.020 mM Yellow 5 dye at lambda max?

(6) Given the data in the table below, what is the concentration of the diluted solution?

stock solution concentration 0.075 M

stock solution absorbance 1.84

diluted solution absorbance 0.78

Reference no: EM132410129

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