Reference no: EM133296879

1. You are interested in learning more about glycogen metabolism and decide to carry out a series of experiments to understand the pathways better.

Both liver and skeletal muscles have large stores of glycogen. First you inhibit liver phosphorylase and observe a drop in blood glucose levels. You then inhibit muscle phosphorylase and do not observe any effect on blood glucose. Explain each result, including why you observe different results by inhibiting enzymes that perform the same function.

2. You are interested in learning more about glycogen metabolism and decide to carry out a series of experiments to understand the pathways better.

Next, you inhibit the branching enzyme. You do not observe a change in total glycogen levels, however you notice that it takes longer to deplete glycogen stores under starvation conditions. Explain why.

3. You are interested in learning more about glycogen metabolism and decide to carry out a series of experiments to understand the pathways better.

Next, you feed cells with UppNHp, an analog of UTP in which linkage between the β and γ phosphate cannot be hydrolyzed. How would you expect this to affect glycogen metabolism, if at all?