Reference no: EM13858415
3.21 A thermodynamic treatment allows predictions of the stability of DNA. The table below lists the standard Gibbs energies, enthalpies,
and entropies of formation at 298 K of short sequences of base pairs as two polynucleotide chains come together:
Sequence 5′-A-G 5′-G-C 5′-T-G
3′-T-C 3′-C-G 3′-A-C
DseqGo/(kJ mol-1) -5.4 -10.5 -6.7
DseqHo/(kJ mol-1) -25.5 -46.4 -31.0
DseqSo(kJ mol-1) -67.4 -118.8 -80.8
To estimate the standard Gibbs energy of formation of a double stranded piece of DNA, DDNAG3, we sum the contributions from the
formation of the sequences and add to that quantity the standard Gibbs energy of initiation of the process, which in the case treated
in this exercise may be set equal to DinitGo = +14.2 kJ mol-1: DDNAGo = DinitGo + DseqGo(sequences)
Similar procedures lead to DDNAHo and DDNASo.
(a) Provide a molecular explanation for the fact that DinitGo is positive and DseqGo negative.
(b) Estimate the standard Gibbs energy, enthalpy, and entropy changes for the following reaction:
5′-A-G-C-T-G-3′ + 5′-C-A-G-C-T- 3′ → 5′-A-G-C-T-G-3′
3′-T-C-G-A-C-5′
Use DinitHo = +2.5 kJ mol-1
and DinitSo = -37.7 J K-1mol-1
(c) Estimate the 'melting' temperature of the piece of DNA shown in part (b).
|
Write a response to the following problems
: In a sample of 12 bank tellers at a local branch office, the 7 tellers who have completed the advanced training program offered by the bank have a lower average error rate in the processing of transactions than the remaining 5 tellers
|
|
What is the character of matrix
: Using its x, y, and z coordinates, construct a 15 × 15 matrix that describes the application of the S4 symmetry operation to [PtCl4]^2-. (b) What is the character of this matrix?
|
|
Distribution of law enforcement personnel and resources
: In law enforcement crime data is used for many different purposes: distribution of law enforcement personnel and resources, funding of initiatives, and on a personal level, even when deciding where to live
|
|
Calculate the steady-state error due to a unit step input
: Given the following block diagram with G(s)=1/(s^2+4s+1), derive the closed-loop transfer function (Y(s))/(R(s)). Calculate the steady-state error due to a unit step input.
|
|
A thermodynamic treatment allows predictions
: 3.21 A thermodynamic treatment allows predictions of the stability of DNA. The table below lists the standard Gibbs energies, enthalpies, and entropies of formation at 298 K of short sequences of base pairs as two polynucleotide chains come together:
|
|
Tricostatin a (tsa) is a protein deacetylase inhibitor
: Tricostatin A (TSA) is a protein deacetylase inhibitor. You know that HDACs remove acetyl groups from lysines. You are interested in identifying how TSA works. You do a number of experiments. You find the level of a protein, called survivin is incre..
|
|
If you take 25 ml of a 500 ppb solution and add sufficient
: If you take 25 mL of a 500 ppb solution and add sufficient water until the volume is 100 mL, and mix thoroughly what is the concentration of the final solution?You dilute 10.0 mL of a 2.50 M solution to 500 mL, what is the final concentration?
|
|
What are the benefits of the law?
: What are the benefits of the law?
|
|
Juice is evaporated in a 2-stage process
: Juice is evaporated in a 2-stage process. Juice containing 6% solids enters the first evaporator. It leaves the first evaporator and enters the second evaporator at 18% solids. 400 kg/hr at 36% solids leaves the second evaporator. Calculate the w..
|