A plasmid dna that was also cut with ecor i

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The restriction enzyme ECOR I cuts DNA leaving 5' overhangs, Pst I leaves 3' overhangs after cutting DNA and SMA I leaves blunt ends on the fragments it produces.Indicate wheter DNA ligase can join the following pieces of DNA.

a) a fragment of human DNA that was generated by cutting with Ecor I and a plasmid DNA that was also cut with Ecor I .

b) a fragment of human DNA that was generated by cutting with Ecor I and a plasmid DNA that was cut with Pst I.

c) a fragment of human DNA that was generated by cutting with Sma I and a plasmid DNA that was also cut with Sma I.

Reference no: EM1340053

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