A highly supercoiled dna molecule is cleaved at one site

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Problem- a highly supercoiled DNA molecule is cleaved at one site by a restriction endonuclease, yielding linear DNA. Predict whether each of the following will increaes, decrease, or remain unchanged as a consequence of this treatment.

1) Second virial coefficient

2) Osmotic pressure at 0.1 mg/ml

3) Limit of pi/C as C --> 0

4) Diffusion coefficient

Explain the each and every step in detail for help

Reference no: EM13690132

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