A highly supercoiled dna molecule is cleaved at one site

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Problem- A highly supercoiled DNA molecule is cleaved at one site by a restriction endonuclease, yielding linear DNA. Predict whether each of the following will increase, decrease or remain unchanged as a consequence of this treatment.

1. Second virial coefficient

2. Osmotic pressure at 0.1 mg/ml

3. Limit of pi/C as C --> 0

4. Diffusion coefficient

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Reference no: EM13689960

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