A destabilizing mutation and stabilizing mutation

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Reference no: EM13762934

1-Suggest a destabilizing mutation and stabilizing mutation to helix 201-216. Briefly explain why your proposed mutations are predicted to be destabilizing and stabilizing, respectively. Provide pictures of your two mutant proteins

2-Assume you have a protein x-ray structure. You are trying to validate the structure before using it in an in silico protein engineering experiment. You notice that the protein structure includes an Arg residues that is fully buried in the interior of the protein. Further analysis indicates that the Arg side chain fails to make any salt bridges or hydrogen bonds with other protein residues. This suggests that there is a problem with the protein structure. Every other validation calculation, however, indicates that the structure is a good one. Suggest a testable hypothesis that can account for the Arg data and support the inference that the protein structure is a good one.

Reference no: EM13762934

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