Analytical tests of glycerol
(i) Acrolein test : When glycerol is heated with KHSO4 a very offensive smell is produced due to creation of acrolein. Its aqueous solvent restores the colour of schiff's reagent and reduces Fehling solution and Tollen's reagent.
(ii) Dunstan's test : A drop of phenolphthalein is added approximately 5 ml of borax solution. The pink colour seems on adding 2-3 drops of glycerol, pink colour goes. The pink colour appears on heating and disappears on cooling again.
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