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Explain Advantages and Disadvantages of Spread Plate Method?
The advantages and disadvantages of spread plate method are highlighted next.
Advantages
1. It is useful for the samples having heat sensitive microbes.
2. No subsurface colonies appear in spread plate so isolation of the organism is easy.
Disadvantages
Volume no greater than 0.1 ml can be spread on the nutrient agar plate because it would not soaken well and may result colonies to coalesce as they form.
Compare and contrast the effect of a deletion in the operator of the lactose operon with one in the operator of the histidine operon.
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